Gene Reporter Systems
Promega ZAP70 Kinase Enzyme System
Recombinant full-length human ZAP70 was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. ZAP70 is a nonreceptor protein tyrosine kinase (part of the Syk/Zap70 family) that is involved in signaling by the T-cell antigen receptor.
Promega UDP-Glo Glycosyltransferase Assay + UDP-GlcNAc, 400 assays
The UDP-Glo Glycosyltransferase Assay is a bioluminescent assay for detecting the activity of glycosyltransferases that use UDP-sugars as donor substrates and release UDP as a product.
Promega UDP-Glo Glycosyltransferase Assay + UDP-Glucose, 200 assays
The UDP-Glo Glycosyltransferase Assay is a bioluminescent assay for detecting the activity of glycosyltransferases that use UDP-sugars as donor substrates and release UDP as a product.
Promega p70S6K Kinase Enzyme System
Recombinant full-length human p70S6K was expressed by baculovirus in Sf9 insect cells using an N-terminal His tag. p70S6K is responsible for the phosphorylation of 40S ribosomal protein S6 and is ubiquitously expressed in human adult tissues.
Promega SYK Kinase Enzyme System
Recombinant full-length human SYK was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. SYK is a non-receptor protein tyrosine kinase that is widely expressed in hematopoietic cells.
Promega GSK3α Kinase Enzyme System
Recombinant full-length human GSK3 alpha was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. GSK3 alpha is a multifunctional protein serine kinase implicated in the control of several regulatory proteins.
Promega EPHA1 Kinase Enzyme System
Recombinant mouse EPHA1 (amino acids 569-end) was expressed by baculovirus in Sf9 insect cells using an N-terminal GST tag. EPHA1 is a member of the Eph family of receptor tyrosine kinases that have been implicated in mediating developmental events.
Promega UDP-Glo Glycosyltransferase Assay + UDP-Glucuronic Acid (UDP-GA), 200 assays
The UDP-Glo Glycosyltransferase Assay is a bioluminescent assay for detecting the activity of glycosyltransferases that use UDP-sugars as donor substrates and release UDP as a product.
Promega Dual-Glo™ Luciferase Assay System
A homogeneous reagent system that enables fast, simple quantitation of both firefly and Renilla luciferase luminescence signals from cells that have not been preconditioned or prelysed.
Promega ADP-Glo™ Kinase Assay
ADP-Glo™ Kinase Assay is a luminescent kinase assay that measures ADP formed from a kinase reaction; ADP is converted into ATP, which is converted into light by Ultra-Glo™ Luciferase. The luminescent signal positively correlates with kinase activity.
Promega ViviRen™ Live Cell Substrate
ViviRen Live Cell Substrate is a uniquely engineered coelenterazine that generates three- to fivefold brighter Renilla luciferase luminescence for super-sensitive quantitation of reporter gene, BRET and RNAi activity.
Promega Ultra Pure UDP-GlcNAc, 100mM, 250microl
UDP-sugar substrates are used with the UDP-Glo Glycosyltransferase Assay to detect the activity of glycosyltransferases that use UDP-sugars as donor substrates and release UDP as a product.
Promega Luciferase Reporter Assays to Study Nuclear Receptors: pBIND-ERα Vector
NR ligand binding domain fused to yeast GAL4 TF DBD in the FN26A (BIND) Flexi Vector or use pBIND-Er∝ and pBIND-GR Vectors. Fusion protein NR activates luc2P reporter controlled by 9X GAL4 UAS in pGL4.35. Use pGL4.36 MMTV LTR for androgen or GC responses.
Promega UDP-Glo Glycosyltransferase Assay + UDP-Galactose, 400 assays
The UDP-Glo Glycosyltransferase Assay is a bioluminescent assay for detecting the activity of glycosyltransferases that use UDP-sugars as donor substrates and release UDP as a product.
Promega Ultra Pure UDP-Galactose, 100mM, 50microl
UDP-sugar substrates are used with the UDP-Glo Glycosyltransferase Assay to detect the activity of glycosyltransferases that use UDP-sugars as donor substrates and release UDP as a product.